To develop a pleural surface fluorescence method to measure ion transport in perfused mouse lungs. 建立一种表面荧光技术测量离子在小鼠离体灌注肺肺泡和毛细血管间的转运。
Methods The pleural mesothelial cell cultured directly from the pleural tissue of BALB/ c mouse and after the digestion of trypsinase were taken respectively. 方法取BALB/c小鼠胸膜组织块直接培养和用胰蛋白酶消化后培养胸膜间皮细胞。